We are seeking a motivated Research Assistant / Associate to join our group of investigators (Lako, Thiele, Sernagor) at Bioscience Institute to assess photoreceptor engraftment and their ability to restore vision in early and advanced stages of Retinitis Pigmentosa using mouse models. The Research fellow will join a team of two other researchers (senior research fellow and research technician) and will specifically contribute to genetic engineering of pluripotent stem cell lines to incorporate a rod reporter, AAV injection in mice and 2-photon imaging + data analyses. As part of the team, the research fellow will also perform behavioural assays of transplanted mice, immunofluorescence and FISH analyses of transplanted retinas.
This MRC funded work follows from our recent work on characterisation and transplantation of CRX-photoreceptors in rd1 mice (PMID: 30681766) and their light responses (PMID: 33657251).
A scientific background on pluripotent stem cell culture and differentiation to retinal organoids and retinal biology will be essential and applicants with record of accomplishment and expertise in gene editing in pluripotent stem cells and retinal organoids are preferred. Importantly experience of animal handling, AAV injection into the visual cortex and/or 2-photon imaging, immunofluorescence analyses of mouse retinas are highly desirable for this post.
You will work under the direction of all three investigators from 3rd January 2023 fulltime for 3.5 years on a fixed term contract.
As part of our commitment to career development for research staff, the University has developed 3 levels of research role profiles. These profiles set out firstly the generic competences and responsibilities expected of role holders at each level and secondly the general qualifications and experiences needed for entry at a particular level.