Post-doc genomic instability of embryonic stem cells (M/F).

Organisation/Company

CNRS

Department

Institut de Génétique Humaine

Research Field

Biological sciences
Biological sciences » Biology

Researcher Profile

Recognised Researcher (R2)

Country

France

Application Deadline

16 Feb 2024 – 23:59 (UTC)

Type of Contract

Temporary

Job Status

Full-time

Hours Per Week

35

Offer Starting Date

1 Apr 2024

Is the job funded through the EU Research Framework Programme?

Not funded by an EU programme

Is the Job related to staff position within a Research Infrastructure?

No

Offer Description

The aim of the project is to understand the molecular bases at the origin of genomic instability of embryonic stem cells. The study will focus on the role of a factor recently identified in the host laboratory involved in this process, its interaction with proteins involved in DNA repair as well as with pluripotency factors in embryonic stem cells. of mice. The candidate will use molecular and cellular biology techniques to study the functional interaction between the DNA repair pathway and pluripotency factors.

-Main tasks
-Cloning and amplification of DNA by PCR
-Protein and chromatin immunoprecipitation from cell lysates
-Expression and purification of recombinant proteins
-Tests of enzymatic activity in vitro
-Immunfluorescence

Secondary tasks:
Trainee supervision (master level)

The Institute of Human Genetics offers an exceptional scientific environment with 22 research teams. One of the major areas of research developed at the institute is genome dynamics and epigenetic control. The main aspects of this research concern DNA replication, maintenance of genome integrity, recombination and meiosis, chromatin and nuclear organization.The project will be developed in collaboration with 4 other groups of the Institute.

Requirements

Research Field

Biological sciences

Education Level

PhD or equivalent

Research Field

Biological sciences

Education Level

PhD or equivalent

Languages

FRENCH

Level

Basic

Research Field

Biological sciences

Years of Research Experience

1 – 4

Research Field

Biological sciences » Biology

Years of Research Experience

1 – 4

Additional Information
Eligibility criteria

Skills

-A very good experience in molecular and cellular biology is required.
-Motivation and passion for science.
-At least one publication of good level (IF> =10) as the first author resulting from the work of thesis.
– Autonomy
-Capacities of organization and communication
– Ability to work in a team
– Knowledge of basic techniques of molecular and cellular biology (western blot, PCR, cloning, immunolabeling).
– Pluripotent stem cells culture
– Chromatin Immunoprecipitation

Additional comments

Do not send applications by email. Candidates whose profile does not correspond to the desired profile are kindly asked not to apply.

Website for additional job details

https://emploi.cnrs.fr/Offres/CDD/UMR9002-CELPRO-014/Default.aspx

Work Location(s)

Number of offers available

1

Company/Institute

Institut de Génétique Humaine

Country

France

City

MONTPELLIER

Geofield

Where to apply

Website

https://emploi.cnrs.fr/Candidat/Offre/UMR9002-CELPRO-014/Candidater.aspx

Contact

City

MONTPELLIER

Website

http://www.igh.cnrs.fr/

STATUS: EXPIRED